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. 2020 Feb 11;12:921–929. doi: 10.2147/CMAR.S239326

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© 2020 Liu et al.

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circHIPK3 served as a sponge for miR-330-5p in PC cells. (A) Bioinformatic analysis to search for miR-330-5p interact with circHIPK3-MUT or circHIPK3-WT. (B) 22 miRNA mimics were co-transfected with the circHIPK3 vector into PANC-1/GEM cells, to identify miRNAs that were able to bind to the circHIPK3 sequence. The line means reduced at least half of the luciferase reporter activities. (C) RNA pull-down assay for the luciferase activity of circHIPK3-MUT or circHIPK3-WT in PANC-1/GEM cells co-transfected with 4 miRNA mimics. (D) RIP assay for the amount of circHIPK3 and miR-330-5p in PANC-1-GEM cells transfected with either sh-circHIPK3 or negative control. (E) qRT-PCR analysis of expression levels of miR-330-5p in PANC-1-GEM and SW 1990-GEM cells transfected with sh-circHIPK3 was upregulated compared with parental control. (F) the expressions levels of miR-330-5p were detected in PC tumor tissues and normal tissues. (G) the expression levels of miR-330-5p in PC tissues with GEM-sensitive compared with GEM-resistant. Data represent mean ± SD. *P < 0.05.