Table 1. Various centrifugation settings.
| Author | Rpm or g | Duration | Outcome |
|---|---|---|---|
| Coleman 13 | 3,000 rpm | 3 min | Lipoaspirate clinically viable |
| Rigotti et al 22 | 2,700 rpm | 15 min | Intact adipocytes very rare |
| Kurita et al 23 |
400
g
700 g 1,200 g 3,000 g 4,200 g |
3 min | Centrifugation at more than 3,000 g significantly damaged ASCs |
| Ferraro et al 18 | Decantation 500 rpm 1,300 rpm 3,000 rpm |
10 min 10 min 5 min 3 min |
Decantation and 500 rpm similar; greater peripheral destruction of adipocytes at 1,300 and 3,000 rpm |
| Pulsfort et al 19 | 1,000 rpm 1,500 rpm 3,000 rpm 5,000 rpm 7,500 rpm 10,000 rpm 15,000 rpm |
5 min | Centrifugation acceleration has no effect on adipocyte viability |
| Hoareau et al 21 |
100
g
400 g 900 g (3,000 rpm) 1,800 g (6,000 rpm) |
1 s, 1 min 1 min 1 min, 3 min 10 min |
Strong centrifugation (900 g , 1800 g ) is deleterious for adipose tissue compared with low centrifugation (100 g , 400 g ) |
| Asilian et al 20 | 3,400 rpm | 1 min | No difference in clinical outcome when compared with filtration/washing |
Abbreviations: ASCs, adipose-derived stem/stromal cells; g , g-force; rpm, revolutions per minute.