Figure 1:

Binge-like ethanol consumption of a 20% (v/v) ethanol solution in male C57BL/6J mice beginning approximately 30 minutes after intraperitoneal (i.p.) injection of two injections of vehicle (VEH-VEH, n = 12), an i.p. injection of vehicle followed by an injection of a 20 mg/kg dose of BUP (VEH-BUP, n = 13), an i.p. injection of vehicle followed by an injection of a 3 mg/kg NAL (VEH-NAL, n = 12), or an i.p. injection of a 3 mg/kg dose of NAL followed by an injection of a 20 mg/kg dose of BUP (NAL-BUP, n = 12). There was a significant effect of drug treatment during the first hour of testing [F(3, 45) = 4.43, p = 0.008), and Bonferroni corrected t-tests indicated that relative to the VEH-VEH group, the NAL-BUP group drank significantly less ethanol (panel A). However, as seen in panel B, there were no group differences in ethanol intake during the second hour of testing [F(3, 45) = 0.67, p > 0.05]. Assessment of blood ethanol concentrations (VEH-VEH = 93.9 + 18.61 mg/dl; VEH-BUP = 88.93 + 15.49 mg/dl; VEHNAL = 44.51 + 9.07 mg/dl; NAL-BUP = 46.55 + 7.33 mg/dl) was statistically significant [F(3, 45) = 3.86, p = 0.015] though Bonferroni corrected t-tests failed to find individual group differences. All data are presented as mean + SEM. *p < 0.05 relative to VEH-VEH group.