ACSL isoform protein abundance in gastrocnemius muscle following either a 12-week low-fat (LFD) or high-fat diet (HFD) in sedentary (SED) and aerobic exercise trained (EX-Tr) mice. Total protein abundance for A) ACSL1, B) ACSL4, C) ACSL5, D) ACSL6, E) with representative western blot images shown. Each representative image was spliced from the same blot to remove groups not used in this study. The effect of diet was analyzed by unpaired students t-test comparing HFD to LFD among sedentary mice. The effect of exercise training was analyzed by unpaired students t-test comparing EX-Tr to SED within each diet condition. Data are presented as mean and standard deviation with individual data points shown. *P≤0.05 vs. LFD, #P≤0.05 vs. SED. n=10 for all conditions.