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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Virology. 2020 Jan 7;542:63–70. doi: 10.1016/j.virol.2020.01.001

Figure 4: TF antagonizes type 1 interferon responses in vitro and in vivo.

Figure 4:

A-B) Peritoneal macrophages were harvested from female C57BL/6 mice and plated in tissue culture for 48 hours to allow adherence. Cultures were washed to remove non-adherent cells and subsequently infected with WT Sindbis or 6K only (MOI =1). RNA was harvested at the indicated timepoints and virus (A) and interferon beta (B) were quantified by qRT-PCR. The housekeeping gene HPRT was used to normalize expression. Data are expressed as mean +/− S.E.M. of fold changes relative to the 2hpi timepoint of WT infected cells to allow pooling of three independent experiments. C) Male and female 3-4 week old C57BL/6 mice were infected with WT Sindbis (n=10) or 6K only (n=10) via intracranial inoculation (1e3 PFU). 24 hours post infection mice were euthanized, brains were harvested, and RNA was isolated. Levels of Sindbis virus and interferon beta RNA were quantified by qRT-PCR. Data are expressed as mean +/− S.E.M. of fold changes relative to the WT infected mice to allow pooling of two independent experiments. D-E) Peritoneal macrophages were harvested from female IFNAR−/− mice and plated and infected as in (A, B). RNA was harvested at the indicated timepoints and virus (D) and interferon beta (E) were quantified by qRT-PCR. The housekeeping gene HPT was used to normalize expression. Data are expressed as mean +/− S.E.M. of fold changes relative to the 2hpi timepoint of WT infected cells to allow pooling of three independent experiments. F) Peritoneal macrophages were harvested from female C57BL/6 mice and plated in tissue culture for 48 hours to allow adherence. Cultures were washed to remove non-adherent cells and subsequently infected with WT or 6K only SINV (MOI =1). At 24hpi, RNA was isolated and samples were assessed for two host transcripts with high turnover rates. Data for Csf-2 and Transferrin receptor p90 are expressed relative to HPRT values on the left y-axis, and HPRT Ct values are shown on the right y-axis. Statistics were performed using Student’s t-test in all cases.