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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Virology. 2019 Dec 30;541:160–173. doi: 10.1016/j.virol.2019.12.011

Figure 8. vIRFs are dispensable for efficient silencing of type I IFN production during de novo lytic infection of iSLK-preRTA cells.

Figure 8.

(A) Setup of the experiment. iSLK-preRTA cells were infected with WT or ΔvIRF KSHV for 48 hours followed by SeV (1 HA/ml) infection for 6 hours. (B) The early interferon response was analyzed by quantifying IFNβ mRNA 6 hours after SeV infection. (C) The late interferon response was analyzed by assessing the amount of IFNα/β secreted into the supernatant by type I IFN reporter bioassay 24 hours after SeV infection. The concentration of type I IFN was determined using an IFNβ standard curve. Error bars represent standard deviation (n=3). T-test was performed between SeV-treated WT and ΔvIRF samples (ND: not detected, ns: non-significant, *p<0.05).