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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: Virology. 2019 Dec 30;541:160–173. doi: 10.1016/j.virol.2019.12.011

Table 1. Primers used for generating recombinant BAC16 clones.

All primers listed are given in 5’ to 3’ orientation.

Oligo name Oligo sequence in 5’ to 3’ orientation Purpose
vIRF1_bacF CACTGGACATTGCGGCGCGAGCTAGTCTGGTTGCGGGACAATGGACTACAAAGACCATGA BAC16–3xFLAG-vIRF1
vIRF1_bacR CCCCAGGCGCCCCAAAAGGGTTCGGTCTTTGGCCTGGGTCTCCACCCTTGTCATCGTCATCCTTGTAATCGATGTCATGAAACCAATTAACCAATTCTGATTAG BAC16–3xFLAG-vIRF1
vIRF1stop_bacR CCCCAGGCGCCCCAAAAGGGTTCGGTCTATTGGCCTGGGTCTCCACCCTTGTCATCGTCATCCTTGTAATCGATGTCATGAAACCAATTAACCAATTCTGATTAG BAC16–3xFLAG-vIRF1 knockout
vIRF2_bacF ACGGAAAAGGTGTTTTGTGTCGTGGCTTTTGCCTAAAAAGATGGACTACAAAGACCATGA BAC16–3xFLAG-vIRF2
vIRF2_bacR TAATAAAGTCCGTGAGCCATTCCGACTCCGTGTAGCGAGGTCCACCCTTGTCATCGTCATCCTTGTAATCGATGTCATGAAACCAATTAACCAATTCTGATTAG BAC16–3xFLAG-vIRF2
vIRF2stop_bacR TAATAAAGTCCGTGAGCCTATTCCGACTCCGTGTAGCGAGGTCCACCCTTGTCATCGTCATCCTTGTAATCGATGTCATGAAACCAATTAACCAATTCTGATTAG BAC16–3xFLAG-vIRF2 knockout
vIRF3–3xF_bacF CGTCATCAAGCTCAGATTGCGTTCAGTTACATGTGATGACGGTGGAATGGACTACAAAGACCATGA BAC16-vIRF3–3xFLAG
vIRF3–3xF_bacR CCACAGCCCGTCAAACCACAGGGACCCTGTTGGCTGACTACTACTTGTCATCGTCATCCTTGTAATCGATGTCATGATCTTTAAACCAATTAACCAATTCTGATTAG BAC16-vIRF3–3xFLAG
vIRF3stop_bacF TGACAGGTCAACATGGCGGGACGCAGGCTTACCTGGATTTGCTAGCGTTTATTGTAGGTGCTTTGGAGGATGACGACGATAAGTAGGG BAC16-vIRF3 knockout
vIRF3stop_bacR CAAAGGATATTTATCAGAGTCCAAAGCACCTACAATAAACGCTAGCAAATCCAGGTAAGCCTGCGTAACCAATTAACCAATTCTGATTAG BAC16-vIRF3 knockout
vIRF4_bacF TGCCTCAAAGACGAACGCCGATCGGTTTCTGTGTCGGACCATGGACTACAAAGACCATGA BAC16–3xFLAG-vIRF4
vIRF4_bacR TAATCCATAACGTGGCCCATTCTGAGCCACCGGCTTTAGGTCCACCCTTGTCATCGTCATCCTTGTAATCGATGTCATGAAACCAATTAACCAATTCTGATTAG BAC16–3xFLAG-vIRF4
vIRF4stop_bacR TAATCCATAACGTGGCCCTATTCTGAGCCACCGGCTTTAGGTCCACCCTTGTCATCGTCATCCTTGTAATCGATGTCATGAAACCAATTAACCAATTCTGATTAG BAC16–3xFLAG-vIRF4 knockout
vIRF2stop_bacF2 ACGGAAAAGGTGTTTTGTGTCGTGGCTTTTGCCTAAAAAGATGCCTCGCTACACGGAGTCAGGATGACGACGATAAGTAGGG vIRF2 knockout in BAC16-QKO
vIRF2stop_bacR2 TAAAGTCCGTGAGCCTATTCCGACTCCGTGTAGCGAGGCATCTTTTTAGGCAAAAGCCACGAACCAATTAACCAATTCTGATTAG vIRF2 knockout in BAC16-QKO
vIRF4stop_bacF2 TGCCTCAAAGACGAACGCCGATCGGTTTCTGTGTCGGACCATGCCTAAAGCCGGTGGCTCAGGATGACGACGATAAGTAGGG vIRF4 knockout in BAC16-QKO
vIRF4stop_bacR2 TCCATAACGTGGCCCTATTCTGAGCCACCGGCTTTAGGCATGGTCCGACACAGAAACCGATAACCAATTAACCAATTCTGATTAG vIRF4 knockout in BAC16-QKO
vIRFdel_bacF CTCCCTCCCATAACAATACGGTGTAGGCATTTTGTATTATCTTTTTAGGCAAAAGCCACGAGGATGACGACGATAAGTAGGG BAC16-ΔvIRF
vIRFdel_bacR ACGGAAAAGGTGTTTTGTGTCGTGGCTTTTGCCTAAAAAGATAATACAAAATGCCTACACAACCAATTAACCAATTCTGATTAG BAC16-ΔvIRF