Table 1.
The information of the antibodies used in the present work.
| Antibody | Source | Dilutions | Company |
|---|---|---|---|
| E-cadherin | Mouse | 1 : 1000 | BD Biosciences, San Jose, USA |
| α-SMA | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| Collagen I | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| Collagen III | Rabbit | 1 : 300 | Wanleibio, Shenyang, China |
| Fibronectin | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| N-cadherin | Rabbit | 1 : 2000 | Huabio, Hangzhou, China |
| SIRT1 | Rabbit | 1 : 500 | Huabio, Hangzhou, China |
| SIRT2 | Rabbit | 1 : 1000 | Huabio, Hangzhou, China |
| SIRT3 | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| SIRT6 | Rabbit | 1 : 2000 | Abcam, Cambridge, UK |
| SOD2 | Rabbit | 1 : 1000 | Wanleibio, Shenyang, China |
| p22phox | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| iNOS | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| Nrf2 | Rabbit | 1 : 1000 | Wanleibio, Shenyang, China |
| GST Pi | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| GSHPX1 | Rabbit | 1 : 1000 | Wanleibio, Shenyang, China |
| TNF-α | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| ICAM-1 | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| IL-1β | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| Cleaved TGF-β1 | Rabbit | 1 : 1000 | Wanleibio, Shenyang, China |
| MMP9 | Rabbit | 1 : 1000 | Wanleibio, Shenyang, China |
| HMGB1 | Rabbit | 1 : 2500 | Huabio, Hangzhou, China |
| RAGE | Rabbit | 1 : 1000 | Bimake, Shanghai, China |
| NLRP3 | Rabbit | 1 : 1500 | Wanleibio, Shenyang, China |
| Cleaved caspase-1 | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| NF-κB p65 | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| P-NF-κB p65 | Rabbit | 1 : 1000 | CST, Boston, USA |
| P-AKT | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| Cleaved caspase-3 | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| Bax | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| Bcl2 | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| LC3 | Rabbit | 1 : 1000 | CST, Boston, USA |
| p62 | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| PERK | Rabbit | 1 : 1000 | Wanleibio, Shenyang, China |
| BIP | Rabbit | 1 : 1500 | Wanleibio, Shenyang, China |
| Chop | Rabbit | 1 : 500 | Wanleibio, Shenyang, China |
| β-Actin | Rabbit | 1 : 5000 | ABclonal, Woburn, USA |