Table 4.

N. ashei venom fractionation program.

Step	Segment	Initial %B	Final %B	FR (mL/min)	CV
Equilibration	Isocratic	0	0	6	3
Sample application	Isocratic	0	0	3	0.5
Column wash	Isocratic	0	0	6	3
Elution	Isocratic	21	21	6	4
	Gradient	21	56	6	30
	Isocratic	56	56	6	3
	Isocratic	100	100	6	7
Column wash	Isocratic	0	0	6	5

B—buffer B (50 mM sodium acetate pH 5 with 1M NaCl), FR—flow rate, CV—column volume.