Figure 3.
Effect of fucoidan with 2-APB, BAPTA/AM or ruthenium red on calcium concentration in the cytosol and mitochondria. (A,B) Flow cytometry of cytosolic Ca2+ with co-treatment of fucoidan with 2-APB, BAPTA/AM, or ruthenium red for 48 h in ES-2 and OV-90 cells. (C,D) Flow cytometry of mitochondrial Ca2+ concentrations in ES-2 and OV-90 cells by the combined treatment of fucoidan and 2-APB, BAPTA/AM or ruthenium red for 48 h. ** p < 0.01 and * p < 0.05 indicate significant effects as compared to the vehicle treated control. # indicates significant effects (p < 0.05) as compared to fucoidan alone.