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. 2020 Jan 9;18(1):45. doi: 10.3390/md18010045

Figure 6.

Figure 6

Stimulation of endoplasmic reticulum (ER) stress sensor, ER–mitochondria tethering proteins, and autophagy regulators by fucoidan in ES-2 and OV-90 cells. (A–F) The stimulation of ER stress sensor proteins, (A) IRE1α, (B) ATF6α, (C) p-PERK, (D) GADD153, (E) p-eIF2α, and (F) GRP78 was observed through western blot analysis in ES-2 and OV-90 cell lines incubated with fucoidan. (G–L) The activation of ER-mitochondria axis-associated proteins, (G) VDAC, (H) IP3R1, (I) IP3R2, (J) GRP75, (K) MFN2, and (L) VAPB was analyzed by western blotting in fucoidan treated ES-2 and OV-90 cells. (M–O) The activation of autophagy-associated proteins, (M) LC3B, (N) BECN1, and (O) ATG5 was observed through western blot in ES-2 and OV-90 cells incubated with fucoidan. The graph of the signals was calculated compared with total signal or α tubulin (TUBA). *** p < 0.001, ** p < 0.01, and * p < 0.05 show significances.