Table 2.
Gene | Primer Sequence (5′ → 3′) | Size (bp) | ||
---|---|---|---|---|
Human | VEGFA (AF022375.1) | Forward | TTGTACAAGATCCGCAGACG | 100 |
Reverse | TCACATCTGCAAGTACGTTCG | |||
VEGFB (BC008818.2) | Forward | CAGAGGAAAGTGGTGTCATGG | 90 | |
Reverse | CATGAGCTCCACAGTCAAGG | |||
VEGFC (NM_005429.5) | Forward | ATGTGGGGAAGGAGTTTGG | 94 | |
Reverse | CCCTCACTATTGCAGCAACC | |||
VEGFD (NM_004469.5) | Forward | TGTAAGTGCTTGCCAACAGC | 96 | |
Reverse | TTTCTTGGAATGGGAACAGC | |||
FLT1 (AF063657.2) | Forward | ATGGTCTTTGCCTGAAATGG | 135 | |
Reverse | TAGAAGCCAGTGTGGTTTGC | |||
FLT4 (AY233383.1) | Forward | GTACATGCCAACGACACAGG | 131 | |
Reverse | TCAGGCTTGTTGATGAATGG | |||
KDR (AF063658.1) | Forward | ACCCACGTTTTCAGAGTTGG | 124 | |
Reverse | TCCAGAATCCTCTTCCATGC | |||
GAPDH (BT006893.1) | Forward | GGCTCTCCAGAACATCATCC | 149 | |
Reverse | TTTCTAGACGGCAGGTCAGG | |||
Zebrafish | p53 (NM_001271820.1) | Forward | GCTTGTCACAGGGGTCATTT | 94 |
Reverse | ACAAAGGTCCCAGTGGAGTG | |||
casp3 (NM_131877.3) | Forward | AAAGGATCCCAGTGGAGGCAGATT | 131 | |
Reverse | TGGTCATGATCTGCAAGAGCTCCA | |||
casp8 (NM_131510.2) | Forward | AGAGAAGGGCACAGTTTTGG | 140 | |
Reverse | CCTGGTTCTCATCTCCTTGG | |||
casp9 (NM_001007404.2) | Forward | CTGCTGTGTGGTCATCATCC | 134 | |
Reverse | GACAGTTCTGGCCATTGAGG | |||
vegfaa (AF016244.1) | Forward | ATTCATACCCAGCAGCTTCG | 137 | |
Reverse | GCAGACAGATGGAGGAGAGC | |||
vegfc (AF466147.1) | Forward | GATGTGGGGAAAGAGTTTGG | 112 | |
Reverse | TGATGTTCCTGCACTGAAGC | |||
flt1 (BC139515.1) | Forward | CTGGTTATTCGGGATGTTGC | 121 | |
Reverse | TTTGGGGCTTCACATTTACC | |||
flt4 (AY833404.1) | Forward | TCACAACTGGATGGATTTGG | 100 | |
Reverse | GCCGACAGTCTTTTCTTTGC | |||
kdr (NM_001024653.2) | Forward | CTTGGCAGCCAGAAATATCC | 116 | |
Reverse | GACGAGCATCTCCTTTACGG | |||
kdrl (NM_131472.1) | Forward | CCTGATCCACAACTGCTTCC | 142 | |
Reverse | CACACGACTCAATGCGTACC | |||
gapdh (BC083506.1) | Forward | TGCTGGTATTGCTCTCAACG | 93 | |
Reverse | GCCATCAGGTCACATACACG |