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. 2020 Jan 9;18(1):45. doi: 10.3390/md18010045

Table 2.

Primer list used in quantitative RT-PCR.

Gene Primer Sequence (5′ → 3′) Size (bp)
Human VEGFA (AF022375.1) Forward TTGTACAAGATCCGCAGACG 100
Reverse TCACATCTGCAAGTACGTTCG
VEGFB (BC008818.2) Forward CAGAGGAAAGTGGTGTCATGG 90
Reverse CATGAGCTCCACAGTCAAGG
VEGFC (NM_005429.5) Forward ATGTGGGGAAGGAGTTTGG 94
Reverse CCCTCACTATTGCAGCAACC
VEGFD (NM_004469.5) Forward TGTAAGTGCTTGCCAACAGC 96
Reverse TTTCTTGGAATGGGAACAGC
FLT1 (AF063657.2) Forward ATGGTCTTTGCCTGAAATGG 135
Reverse TAGAAGCCAGTGTGGTTTGC
FLT4 (AY233383.1) Forward GTACATGCCAACGACACAGG 131
Reverse TCAGGCTTGTTGATGAATGG
KDR (AF063658.1) Forward ACCCACGTTTTCAGAGTTGG 124
Reverse TCCAGAATCCTCTTCCATGC
GAPDH (BT006893.1) Forward GGCTCTCCAGAACATCATCC 149
Reverse TTTCTAGACGGCAGGTCAGG
Zebrafish p53 (NM_001271820.1) Forward GCTTGTCACAGGGGTCATTT 94
Reverse ACAAAGGTCCCAGTGGAGTG
casp3 (NM_131877.3) Forward AAAGGATCCCAGTGGAGGCAGATT 131
Reverse TGGTCATGATCTGCAAGAGCTCCA
casp8 (NM_131510.2) Forward AGAGAAGGGCACAGTTTTGG 140
Reverse CCTGGTTCTCATCTCCTTGG
casp9 (NM_001007404.2) Forward CTGCTGTGTGGTCATCATCC 134
Reverse GACAGTTCTGGCCATTGAGG
vegfaa (AF016244.1) Forward ATTCATACCCAGCAGCTTCG 137
Reverse GCAGACAGATGGAGGAGAGC
vegfc (AF466147.1) Forward GATGTGGGGAAAGAGTTTGG 112
Reverse TGATGTTCCTGCACTGAAGC
flt1 (BC139515.1) Forward CTGGTTATTCGGGATGTTGC 121
Reverse TTTGGGGCTTCACATTTACC
flt4 (AY833404.1) Forward TCACAACTGGATGGATTTGG 100
Reverse GCCGACAGTCTTTTCTTTGC
kdr (NM_001024653.2) Forward CTTGGCAGCCAGAAATATCC 116
Reverse GACGAGCATCTCCTTTACGG
kdrl (NM_131472.1) Forward CCTGATCCACAACTGCTTCC 142
Reverse CACACGACTCAATGCGTACC
gapdh (BC083506.1) Forward TGCTGGTATTGCTCTCAACG 93
Reverse GCCATCAGGTCACATACACG