Effects of AHG supplementation on AMPK signaling pathway in the livers of insulin resistant mice induced with HFD. C57BL/6J mice were fed with HFD for 12 weeks and treated with low, medium and high doses (20, 50 and 100 mg/kg/day, respectively) of AHG for eight weeks. (A) The protein expression of p-ACC (S79), ACC, p-AMPKα (T172) and AMPKα were measured by Western blot. (B) Quantification of p-ACC (S79)/ACC and p-AMPKα (T172)/ AMPKαwas performed by Image J. Data are showed as mean ± SD, n = 3; * p < 0.5, **p < 0.1, ***p < 0.01 vs. LFD group, #
p < 0.5, ##
p < 0.1, ###
p < 0.01 vs. HFD group.