Table 2.
The specific activity of TsAly7B and truncated mutants.
| Protein | Specific Activity (U/mg) |
Molecular Weight (kDa) |
Specific Activity (U/nmol) |
|---|---|---|---|
| TsAly7B-FL | 488.8 ± 12.4 | 64.79 | 31.6 ± 0.8 |
| TsAly7B-TM1 | 869.1 ± 9.1 | 51.35 | 44.6 ± 0.5 |
| TsAly7B-TM2 | 1147.2 ± 19.7 | 52.66 | 60.4 ± 1.0 |
| TsAly7B-TM3 | 238.4 ± 5.6 | 33.78 | 8.0 ± 0.2 |
Values were determined at the optimal pH and temperature in the presence of 0.5 M NaCl.