Table 5.
Chemical fingerprints of the RYR dietary supplements analyzed by UHPLC and quantitative determination of the monacolins identified (mg per capsule or tablet).
| DS | Monacolins 1 | Total M 2 | MK+MKA (%) | MK/MKA | %DiMK/TotalM | Azaphilones 3 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MJA | MJ | MN | MLA | MX | MKA | ML | CP | MK | DiMK | DeML | DeMK | ||||||
| 1 | - | - | - | - | - | 0.49 | - | - | 1.33 | - | - | 0.02 | 1.84 | 1.82 (98.9) | 2.7 | - | Mocusp, Mop B, Mo |
| 2 | - | - | - | 0.04 | - | 0.82 | 0.20 | 0.03 | 6.77 | 0.21 | 0.05 | 0.68 | 8.80 | 7.59 (86.3) | 8.3 | 2.4 | |
| 3 | - | - | - | - | - | 0.23 | 0.04 | 0.01 | 2.16 | 0.04 | 0.01 | 0.13 | 2.62 | 2.39 (91.2) | 9.4 | 1.5 | |
| 4 | - | 0.01 | 0.02 | 0.01 | - | 0.10 | 0.04 | 0.03 | 0.48 | 0.11 | 0.02 | 0.29 | 1.11 | 0.58 (52.3) | 4.8 | 9.9 | Mocusp |
| 5 | - | 0.01 | 0.02 | 0.02 | - | 0.50 | 0.05 | 0.04 | 1.80 | 0.20 | 0.02 | 0.38 | 3.04 | 2.30 (75.7) | 3.6 | 6.6 | Rubro, Moco, Mocusp, Mop B, Mo, Mof B, Mop A, Anka |
| 6 | - | - | - | 0.03 | - | 0.31 | 0.08 | 0.03 | 2.05 | 0.11 | 0.03 | 0.27 | 2.91 | 2.36 (81.1) | 6.6 | 3.8 | Mop B |
| 7 | - | 0.06 | - | 0.06 | - | 0.37 | 0.19 | 0.08 | 2.22 | 0.20 | 0.06 | 0.85 | 4.09 | 2.59 (63.3) | 6.0 | 4.9 | |
| 8 | - | 0.02 | 0.09 | 0.12 | - | 2.13 | 0.18 | 0.28 | 6.26 | 0.59 | - | 0.16 | 9.83 | 8.39 (85.4) | 2.9 | 6.0 | Cit, Rubro, Mocusp, Mof A, Mop B, Mo |
| 9 | - | - | - | - | - | 0.13 | - | - | 4.17 | - | - | 0.28 | 4.58 | 4.30 (93.9) | 32.1 | - | Mo, Anka |
| 10 | - | 0.02 | - | 0.01 | - | 0.96 | 0.02 | 0.04 | 0.85 | 0.10 | 0.01 | 0.31 | 2.32 | 1.81 (78.0) | 0.9 | 4.3 | Cit, Rubro, Mocusp, Mop B |
| 11 | - | - | - | - | - | - | - | - | 0.07 | - | - | - | 0.07 | 0.07 (100) | - | - | Rubro, Moco, Mop B, Mo, Mop A, Anka |
| 12 | - | - | - | - | - | - | - | - | 0.08 | - | - | - | 0.08 | 0.08 (100) | - | - | Rubro, PP-R, Moco, Mop B, Mo, Mof B, Mop A, Anka |
| 13 | - | - | - | - | - | 0.62 | - | - | 23.18 | - | - | 0.04 | 23.84 | 23.80 (99.8) | 37.4 | - | |
| 14 | 0.03 | 0.05 | 0.07 | 0.04 | - | 0.27 | 0.18 | 0.08 | 2.19 | 0.23 | 0.06 | 0.56 | 3.76 | 2.46 (65.4) | 8.1 | 6.1 | Mocusp, Mop B, Mo |
| 15 | - | - | - | 0.19 | - | 1.33 | 0.86 | 0.58 | 8.27 | 1.16 | 0.16 | 1.44 | 13.99 | 9.60 (68.6) | 6.2 | 8.3 | Rubro, Mocusp, Mof A, Mop B, Mo |
| 16 | - | - | - | - | - | - | 0.02 | 0.02 | 0.33 | 0.01 | - | 0.03 | 0.41 | 0.33 (80.5) | - | 2.4 | Rubro, PP-R, Moco, Mop B, Mo, Mof B, Mop A, Anka |
| 17 | - | - | - | 0.03 | - | 0.16 | 0.12 | 0.16 | 1.09 | 0.13 | 0.02 | 0.15 | 1.86 | 1.25 (67.2) | 6.8 | 7.0 | Mocusp, Mof A, Mop B, Mo |
| 18 | - | - | - | - | - | 0.98 | - | - | 6.44 | 0.13 | - | 0.38 | 7.93 | 7.42 (93.6) | 6.6 | 1.6 | Mocusp, Mop B, Mo |
| 19 | - | 0.01 | - | 0.01 | - | 0.05 | 0.03 | 0.01 | 0.25 | 0.02 | 0.01 | 0.11 | 0.50 | 0.30 (60.0) | 5.0 | 4.0 | |
| 20 | - | - | - | - | - | 0.48 | - | - | 1.29 | - | - | 0.02 | 1.79 | 1.77 (98.9) | 2.7 | - | Mocusp, Mop B, Mo |
| 21 | - | 0.01 | 0.01 | 0.01 | - | 0.07 | 0.02 | - | 0.24 | 0.02 | 0.01 | 0.12 | 0.51 | 0.31 (60.8) | 3.4 | 3.9 | |
| 22 | - | - | - | 0.02 | - | 0.68 | 0.03 | 0.08 | 1.77 | 0.17 | 0.01 | 0.37 | 3.13 | 2.45 (78.3) | 2.6 | 5.4 | Rubro, Mocusp, Mop B, Mo |
| 23 | - | - | - | 0.04 | 0.01 | 0.68 | 0.05 | 0.10 | 8.47 | 0.17 | 0.03 | 0.23 | 9.78 | 9.15 (93.6) | 12.5 | 1.7 | Rubro, Mocusp, Mop B, Mo |
| 24 | - | - | - | - | - | 5.50 | - | - | 5.21 | - | - | 0.07 | 10.78 | 10.71 (99.4) | 0.95 | - | |
| 25 | - | 0.09 | 0.10 | 0.11 | 0.04 | 3.56 | 0.14 | 0.29 | 5.69 | 0.67 | 0.03 | 0.56 | 11.28 | 9.25 (82.0) | 1.6 | 5.9 | Rubro, Mocusp, Mof A, Mop B, Mo |
| 26 | - | - | - | 0.035 | - | 0.39 | 0.10 | 0.04 | 3.52 | 0.15 | 0.035 | 0.48 | 4.75 | 3.91 (82.3) | 9.0 | 3.2 | Mocusp |
| 27 | - | - | - | - | - | 0.61 | - | - | 2.68 | - | - | 0.09 | 3.38 | 3.29 (97.3) | 4.4 | - | Mop B, Mo |
| 28 | - | 0.03 | 0.04 | 0.04 | - | 0.41 | 0.07 | 0.05 | 0.98 | 0.15 | 0.02 | 0.32 | 2.11 | 1.39 (65.9) | 2.4 | 7.1 | Mop B |
| 29 | - | - | - | - | - | 0.01 | - | - | 0.08 | - | - | 0.04 | 0.13 | 0.09 (69.2) | 8.0 | - | |
| 30 | - | 0.02 | 0.03 | 0.02 | - | 0.37 | 0.05 | 0.02 | 2.19 | 0.07 | 0.02 | 0.30 | 3.09 | 2.56 (82.8) | 5.9 | 2.3 | Mop B |
| 31 | - | - | - | - | - | 0.31 | 0.04 | 0.03 | 0.85 | 0.07 | 0.01 | 0.12 | 1.43 | 1.16 (81.1) | 2.7 | 4.9 | Rubro, Mop B, Mo, Anka |
1 Monacolins are listed in increasing order of elution times. MJ, MN, MX, ML, MK: monacolins J, N, X, L, K; MJA, MLA, MKA: monacolins J, L, K in hydroxyl acid forms; CP: compactin; DiMK: dihydromonacolin K; DeML, DeMK: dehydromonacolins L,K. 2 TotalM: total monacolins. 3 Azaphilones are listed in increasing order of elution times. Anka: ankaflavin; Cit: citrinin; Mo: monascin; Moco: monascorubramine; Mocusp: monascuspiloin; Mof A, Mof B: monasfluore A, monasfluore B; Mop A, Mop B: monaphilone A, monaphilone B; PP-R: 7-(2-hydroxyethyl)-monascorubramine; Rubro: rubropunctamine.