Table 1.
MS/MS parameters for 17 target compounds.
| No. | Compounds | Ion Mode | RT (min) | Precursor Ion | Cone Voltage (V) | Product ion 1 Q | Collision Energy (eV) | Product Ion 2 I | Collision Energy (eV) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 3′-Hydroxydaidein | ESI- | 4.97 | 269 | 52 | 213 | 28 | 241 | 24 |
| 2 | Butein | ESI- | 5.12 | 271 | 35 | 135 | 20 | 253 | 17 |
| 3 | Daidzein | ESI- | 5.95 | 253 | 51 | 208 | 31 | 224 | 26 |
| 4 | Liquiritigenin | ESI- | 6.23 | 255 | 40 | 135 | 15 | 119 | 22 |
| 5 | Eriodictyol | ESI- | 6.24 | 287 | 38 | 151 | 17 | 135 | 24 |
| 6 | Butin | ESI- | 7.59 | 271 | 39 | 135 | 31 | 91 | 37 |
| 7 | Naringenin | ESI- | 7.94 | 271 | 40 | 151 | 20 | 119 | 24 |
| 8 | Genistein | ESI- | 8.04 | 269. | 48 | 133 | 32 | 181 | 28 |
| 9 | Tectorigenin | ESI- | 8.30 | 2989 | 40 | 284 | 19 | 240 | 22 |
| 10 | Alpinetin | ESI- | 8.65 | 269 | 44 | 165 | 20 | 227 | 21 |
| 11 | Isoliquiritigenin | ESI- | 9.62 | 255 | 35 | 135 | 15 | 119 | 23 |
| 12 | Formononetin | ESI- | 10.08 | 267 | 45 | 252 | 22 | 223 | 25 |
| 13 | Dalbergin | ESI- | 10.09 | 267 | 38 | 180 | 27 | 252 | 18 |
| 14 | 3′-O-methylviolanone | ESI- | 10.62 | 329 | 46 | 135 | 38 | 299 | 36 |
| 15 | Sativanone | ESI- | 11.10 | 299 | 46 | 135 | 37 | 269 | 32 |
| 16 | Pinocembrin | ESI- | 12.47 | 255 | 42 | 107 | 25 | 171 | 25 |
| 17 | Prunetin | ESI- | 12.97 | 283 | 45 | 268 | 21 | 239 | 26 |
| 18 | Rutin (IS) | ESI- | 4.05 | 609 | 62 | 300 | 52 | 271 | 50 |
Q: transitions for quantification; I: transitions for identification.