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. 2020 Jan 10;25(2):288. doi: 10.3390/molecules25020288

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antioxidant activity of 50% ethanolic extract of C. collinum extract, measured by ABTS (A) and DPPH assays (B). CCL EtOH 50% showed significant concentration-dependent antioxidant activity compared to control (pure ABTS or DPPH solution incubated with a blank). Trolox 6.25 µg/mL was used as a positive control. The two diagrams C and D show that the two main compounds of the extract had significantly higher antioxidant activity than CCL EtOH 50% in both the ABTS (C) and the DPPH assays (D) and thus most likely significantly contributed to the overall antioxidant activity of the extract; values in (C) and (D) are given in Trolox Equivalent Antioxidative Capacity (TEAC). n = 3 replicates, data presented as mean ± SEM, significant for * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.001 compared to the control in ordinary one-way ANOVA (analysis of variance).

Antioxidant activity of 50% ethanolic extract of C. collinum extract, measured by ABTS (A) and DPPH assays (B). CCL EtOH 50% showed significant concentration-dependent antioxidant activity compared to control (pure ABTS or DPPH solution incubated with a blank). Trolox 6.25 µg/mL was used as a positive control. The two diagrams C and D show that the two main compounds of the extract had significantly higher antioxidant activity than CCL EtOH 50% in both the ABTS (C) and the DPPH assays (D) and thus most likely significantly contributed to the overall antioxidant activity of the extract; values in (C) and (D) are given in Trolox Equivalent Antioxidative Capacity (TEAC). n = 3 replicates, data presented as mean ± SEM, significant for * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.001 compared to the control in ordinary one-way ANOVA (analysis of variance).