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. 2019 Dec 18;18(1):3. doi: 10.3390/md18010003

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of P. tricornutum and O. tauri extracts on mRNA expression of IL1-β in endothelial HMEC-1 cells. mRNA expression of IL1-β was analyzed using RT-qPCR. Data are expressed relatively to mRNA levels of IL1-β found in corresponding control cells (DMSO), arbitrarily set to 1 unit. HMEC-1 cells were exposed to vehicle (DMSO) or treated with 2 μM B[a]P, or 0.1, 1 and 5 µg/mL P. tricornutum (PT) extract (a) or O. tauri (OT) extract (b), or with a combination of the toxicant and the extract for 24 h. Results are represented as mean values ± SD from at least 3 independent experiments. Mean values assigned with different letters are significantly different (p < 0.05) with a < b < c.