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. 2019 Dec 18;18(1):3. doi: 10.3390/md18010003

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of B[a]P and O. tauri extracts on the EVs released by endothelial HMEC-1 cells. HMEC-1 cells were exposed to vehicle (DMSO), 2 µM of B[a]P, 0.1 µM/mL of O. tauri (OT) extracts or with a combination of the toxicant and the extract for 24 h. Total EVs were isolated by ultracentrifugation and analyzed by Nanoparticle Tracking Analysis (NTA). (a) EV production released per HMEC-1 cells exposed with B[a]P or/and OT. (b) Representative size distribution profile by NTA of EVs produced by endothelial cells. Results are represented as mean values ± SD from at least 3 independent experiments. Mean values assigned with different letters are significantly different (p < 0.05) with a < b.