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. 2020 Jan 16;25(2):367. doi: 10.3390/molecules25020367

Figure 2.

Figure 2

The effects of IMiDs on the metabolism of normal monocytes; a presentation of statistically significant effects of LPS and IMiDs on the OCR:ECAR ratio, (A–C) is OCR:ECAR ratio at basal levels and (D–H) at maximal respiration. Normal monocytes derived from 10 different healthy individuals were cultured with and without LPS 1 ng/mL and/or with and without IMiDs 5 μg/mL in the presence of LPS. Metabolism was analyzed using the XF Mito Stress Test assay and the Seahorse XF 96 cell analyzer. The figure presents the results for all statistically significant comparisons. The culture conditions that were compared in each of the statistical analyses/diagrams are indicated on the x-axis, the OCR:ECAR ratio is indicated on the y-axis and the corresponding p-values from the statistical analyses (Wilcoxon test for paired samples) are indicated at the top of each diagram. We present the results from analysis of basal (A–C) and maximal respiration (D–H). Each diagram compares the results for (i) cultures prepared with medium alone versus LPS (A,D); (ii) cultures prepared with LPS without and with an IMiD (B,F,G), and (iii) cultures prepared with two different IMiDs but otherwise with similar culture conditions (E,H).