Figure 4.

Analysis of monocyte cytokine release during in vitro culture; a summary of the overall results. Normal monocytes were cultured in medium alone or in the presence of LPS 1 ng/mL, or IMiD 5 μg/mL for 24 h before supernatants were harvested and the supernatant levels of the 14 soluble mediators determined. Each of the diagrams/figures present the level for cultures prepared in (from the bottom to the top of the figure) (i) medium alone (ctr), (ii) LPS + DMSO alone, (iii) each of the IMiDs—thalidomide, lenalidomide or pomalidomide—in combination with either thalidomide, lenalidomide, or pomalidomide. The diagrams show the results for each individual soluble mediator. The results are presented as the median, box (i.e., 25–75 percentiles), and whiskers (5–95 percentiles). The Wilcoxon test for paired samples was used for all statistical comparisons, and p-values < 0.05 were regarded as statistically significant. All statistically significant comparisons are indicated in the figure (* 0.01 < p < 0.05, ** p < 0.01, *** p < 0.001).