Table 2.

Relative expression of nAChR subunits protein (fold) in breast cancer cell lines compared with normal cell line HS578BST.

Cell Lines	nAChR Subunits (Protein Level)
Name	α3	α4	α5	α6	α7	α9	α10	β2	β3	β4
MDA-MB-453	ns	ns	ns	ns	ns	ns	ns	ns	ns	ns
BT-549	ns	ns	ns	ns	ns	+++	ns	ns	ns	ns
MDA-MB-361	ns	+	+	ns	ns	+++	ns	ns	ns	ns
HS578T	ns	ns	ns	ns	ns	+++	ns	ns	+++	ns
Bcap-37	ns	ns	ns	ns	ns	+++	ns	ns	++	ns
HCC1395	ns	ns	ns	ns	ns	ns	ns	ns	ns	ns
BT-483	ns	ns	+++	++	+	+++	ns	ns	+++	ns
SK-BR-3	ns	+	+	ns	ns	+++	ns	ns	ns	+
BT-20	ns	++	+	+	++	+++	ns	ns	+++	ns
ZR-75-30	++	+	++	+	+	+++	+++	++	ns	++
HCC1806	+++	+++	+++	+++	ns	+++	++	ns	++	ns
MCF-7	ns	ns	ns	ns	ns	+++	++	ns	ns	ns
AU565	+++	+++	+++	+++	+++	+++	+++	++	ns	+++
HCC1937	+++	ns	ns	ns	ns	+++	ns	ns	++	ns
MDA-MB-231	++	++	ns	ns	++	+++	ns	ns	ns	++
MDA-MB-157	+++	+++	++	+++	+++	+++	+++	ns	+++	+++
BT474	+++	ns	++	ns	+	+++	ns	ns	++	+
HS578BST	−	−	−		−	−	−	−	−	−

Comparisons of differently expressed of nAChRs in normal control cells (HS578BST) and breast cancer cells. +, ++ and +++ represent upregulation of expression (p < 0.05, 0.01 and 0.001, respectively); ns represented no significant difference.