Table 1.
Primer design for real-time PCR.
| Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
| GAPDH | CGAGATCCCTCCAAAATCAA | TTCACACCCATGACGAACAT |
| HMGCR | CTCCTCCTTACTCGATAC | TAGATACACCACGCTCAT |
| SMYD3 | CCCAGTATCTCTTTGCTCAATCAC | TTACGGGTGTTGAAGGT |
| CYP7A1 | CAGAAGCATAGACCCAAGTGAT | TCGGTAGCAGAAGGCATACATC |
| GLP-1 | GATTCAGTCCCAGGCAGCGTAT | CTTTCTTGATCTTGGCGGGTGTT |
| mHMGCR | TTATGTCTTTAGGCTTGGTC | ACTCAGGGTAATCACTTGC |
| mGAPDH | ATTCAACGGCACAGTCAAGG | GCAGAAGGGGCGGAGATGA |
| mSMYD3 | CTGCTTTGAGTGTGACT | CTGGTAGATGTTGATGT |
| Fragment I | CGCTGATTTGGGTCTATG | GTGCGTTCCTTCTGCTCT |
| Fragment II | ATGGGTAAATCTCGGGAA | GGAGCGTGAGGGAAAACG |
| Fragment III | GCAGGCCCTAGTGCTGGG | AAGAGAGGATCGTTCGAT |
(m: mouse).