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. 2019 Dec 30;18(1):34. doi: 10.3390/md18010034

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative 2-DE gels showing total and carbonylated protein profiles of liver detected in HFHS diets. Coomassie-stained 2-DE gels from (A) HFHS-CONTROL, (B) -FOM, (C) -GSE, and (D) -FOM&GSE. FTSC-stained 2-DE gels from (E) HFHS-CONTROL, (F) -FOM, (G) -GSE, and (H) -FOM&GSE. Numbered protein spots represent carbonylated proteins confidently identified, and they are listed in Table 3. Images are representatives of three independent labelling experiments performed in triplicates. HFHS: high-fat and high-sucrose diet; GSE: grape seed polyphenols extract; FOM: fish oil mixture.