Table 3.
Carbonylated proteins identified from 2-DE gels of both high-fat and high-sucrose (HFHS) (Figure 5) and standard (STD) (Figure 6) diets. Spots of interest were identified by LC-ESI-IT-MS/MS as described in the Materials and Methods Section. Spot N° refers to numbered spot on Figure 5 and Figure 6.
| Spot N° | UniprotKB Accession | Protein Description | Gene Name | Subcellular Localization | Avg. Mass (Da) | Coverage (%) | #Peptides (#Unique) |
|---|---|---|---|---|---|---|---|
| 1 | P07756 | Carbamoyl-phosphate synthase [ammonia] mitochondrial | Cps1 | Mitochondria | 164,579 | 70 | 73 (69) |
| 2 | P12346 | Serotransferrin | Tf | Extracellular region or secreted | 76,346 | 38 | 26 (26) |
| 3 | P02770 | Serum albumin | Alb | Extracellular region or secreted | 68,731 | 42 | 45 (45) |
| 4 | P50137 | Transketolase | Tkt | Endoplasmic reticulum/Peroxisome | 67,644 | 49 | 43 (43) |
| 5,6 | P04762 | Catalase | Cat | Peroxisome | 59,757 | 27 | 18 (18) |
| 7 | P63039 | 60 kDa heat shock protein mitochondrial | Hspd1 | Mitochondria | 60,956 | 31 | 21 (21) |
| 8 | P04785 | Protein disulfide-isomerase | P4hb | Endoplasmic reticulum | 56,951 | 38 | 25 (25) |
| 9 | P11884 | Aldehyde dehydrogenase mitochondrial | Aldh2 | Mitochondria | 56,488 | 31 | 24 (24) |
| 10 | P85968 | 6-phosphogluconate dehydrogenase decarboxylating | Pgd | Mitochondria | 53,236 | 20 | 10 (10) |
| 11 | P60711 | Actin cytoplasmic 1 | Actb | Cytoskeleton | 41,737 | 56 | 49 (24) |
| 12 | P15650 | Long-chain specific acyl-CoA dehydrogenase mitochondrial | Acadl | Mitochondria | 47,873 | 36 | 24 (24) |
| P32755 | 4-hydroxyphenylpyruvate dioxygenase | Hpd | Endoplasmic reticulum | 45,112 | 45 | 24 (24) | |
| Q03248 | Beta-ureidopropionase | Upb1 | Cytoplasm | 44,042 | 28 | 12 (12) | |
| 13 | P09034 | Argininosuccinate synthase | Ass1 | Cytosol | 46,496 | 54 | 48 (48) |
| 14 | P16617 | Phosphoglycerate kinase 1 | Pgk1 | Cytoplasm | 44,538 | 20 | 7 (7) |
| P13437 | 3-ketoacyl-CoA thiolase mitochondrial | Acaa2 | Mitochondria | 41,871 | 41 | 19 (19) | |
| 15 | P25093 | Fumarylacetoacetase | Fah | Cytosol/Extracellular region or secreted | 45,976 | 10 | 4 (4) |
| 16 | P06757 | Alcohol dehydrogenase 1 | Adh1 | Cytoplasm | 39,645 | 46 | 28 (24) |
| 17 | P00507 | Aspartate aminotransferase mitochondrial | Got2 | Mitochondria | 47,314 | 43 | 31 (30) |
| 18 | P00481 | Ornithine carbamoyltransferase mitochondrial | Otc | Mitochondria | 39,886 | 27 | 9 (9) |
| 19 | Q03336 | Regucalcin | Rgn | Cytoplasm | 33,390 | 53 | 27 (27) |
| 20 | P23457 | 3-alpha-hydroxysteroid dehydrogenase | Akr1c9 | Cytoplasm | 37,028 | 40 | 20 (12) |
| 21 | Q497B0 | Omega-amidase NIT2 | Nit2 | Cytoplasm | 30,701 | 28 | 10 (10) |