Table 4.
Carbonylation index calculated for each protein in high-fat and high-sucrose (HFHS) diets. Spot N° refers to numbered spot on Figure 5.
| CARBONYLATION INDEX 1 | FOLD CHANGE2 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Spot N° | Protein Description | Gene Name | HFHS-C | HFHS-FOM | HFHS-GSE | HFHS-FOM&GSE | HFHS-FOM/HFHS-C | HFHS-GSE/HFHS-C | HFHS-FOM&GSE/HFHS-C |
| 1 | Carbamoyl-phosphate synthase [ammonia] mitochondrial | Cps1 | 0.27 (0.01) | 0.32 (0.14) | 0.22 (0.11) | 0.31 (0.06) | 1.19 | 0.81 | 1.15 |
| 2 | Serotransferrin | Tf | 0.24 (0.05) | 0.53 (0.25) | 0.19 (0.10) | 0.47 (0.04) *# | 2.21 | 0.79 | 1.96 |
| 3 | Serum albumin | Alb | 1.18 (0.04) | 0.50 (0.18) *# | 1.33 (0.52) & | 0.66 (0.12) * | 0.42 | 1.13 | 0.56 |
| 4 | Transketolase | Tkt | 0.64 (0.13) | 0.67 (0.01) | 0.52 (0.22) | 0.53 (0.52) | 1.05 | 0.81 | 0.83 |
| 5,6 | Catalase | Cat | 1.33 (0.43) | 0.93 (0.50) | 0.77 (0.14) * | 0.57 (0.30) * | 0.70 | 0.58 | 0.43 |
| 7 | 60 kDa heat shock protein mitochondrial | Hspd1 | 0.55 (0.07) | 0.60 (0.12) | 1.45 (0.82) | 0.31 (0.03) *& | 1.09 | 2.64 | 0.56 |
| 8 | Protein disulfide-isomerase | P4hb | 0.76 (0.15) | 0.85 (0.08) | 0.98 (3.42) | 0.93 (0.62) | 1.12 | 1.29 | 1.22 |
| 9 | Aldehyde dehydrogenase mitochondrial | Aldh2 | 0.61 (0.12) | 0.17 (0.08) *# | 2.38 (0.26) *&$ | 0.54 (0.23) # | 0.28 | 3.90 | 0.89 |
| 10 | 6-phosphogluconate dehydrogenase decarboxylating | Pgd | 1.01 (0.02) | 3.98 (1.20) *$ | 1.31 (1.20) | 0.35 (0.01) *& | 3.94 | 1.30 | 0.35 |
| 11 | Actin cytoplasmic 1 | Actb | 0.11 (0.05) | 0.43 (0.14) *# | 0.15 (0.09) | 0.69 (0.27) *# | 3.91 | 1.36 | 6.27 |
| 12 | Long-chain specific acyl-CoA dehydrogenase mitochondrial | Acadl | 1.89 (0.35) | 0.99 (0.18) *$ | 0.82 (0.13) *$ | 0.37 (0.01) *&# | 0.52 | 0.43 | 0.20 |
| 4-hydroxyphenylpyruvate dioxygenase | Hpd | ||||||||
| Beta-ureidopropionase | Upb1 | ||||||||
| 13 | Argininosuccinate synthase | Ass1 | 0.75 (0.42) | 0.42 (0.13) | 0.35 (0.17) | 0.25 (0.26) | 0.56 | 0.47 | 0.33 |
| 14 | Phosphoglycerate kinase 1 3-ketoacyl-CoA thiolase mitochondrial |
Pgk1 Acaa2 |
0.22 (0.10) | 0.33 (0.09) | 0.13 (0.12) | 0.36 (0.01) | 1.50 | 0.59 | 1.64 |
| 15 | Fumarylacetoacetase | Fah | 0.80 (0.76) | 0.55 (0.37) | 0.55 (0.25) | 0.39 (0.01) | 0.69 | 0.69 | 0.49 |
| 16 | Alcohol dehydrogenase 1 | Adh1 | 1.21 (0.14) | 1.69 (0.98) | 1.55 (0.55) | 1.23 (0.21) | 1.40 | 1.28 | 1.02 |
| 17 | Aspartate aminotransferase mitochondrial | Got2 | 1.41 (0.61) | 1.01 (0.89) | 4.46 (0.94) *& | 2.63 (1.02) | 0.72 | 3.16 | 1.87 |
| 18 | Ornithine carbamoyltransferase mitochondrial | Otc | 0.86 (0.18) | 0.61 (0.06) *$ | 0.26 (0.22) * | 0.32 (0.01) *& | 0.71 | 0.30 | 0.37 |
| 19 | Regucalcin | Rgn | 0.18 (0.25) | 0.29 (0.13) | 0.51 (0.23) | 0.29 (0.15) | 1.61 | 2.83 | 1.61 |
| 20 | 3-alpha-hydroxysteroid dehydrogenase | Akr1c9 | 0.20 (0.07) | 0.19 (0.14) | 0.55 (0.15) *& | 0.30 (0.23) | 0.95 | 2.75 | 1.50 |
| 21 | Omega-amidase NIT2 | Nit2 | 1.88 (0.29) | 4.76 (1.20) *#$ | 1.76 (0.18) &$ | 0.79 (0.01) *&# | 2.53 | 0.94 | 0.42 |
1 Results are means (standard deviation) obtained from the densitometric analysis of the 2-DE gels represented in Figure 5 and Figure 6. Data (n = 3) correspond to three independent 2-DE experiments performed in triplicates. * P < 0.05 vs. HFHS-C; & P < 0.05 vs. HFHS-FOM; # P < 0.05 vs. HFHS-GSE; $ P < 0.05 vs. HFHS-FOM&GSE. Comparisons were performed using the one-way ANOVA and Tukey post hoc tests. 2 Fold change of carbonylation index in comparison to HFHS-C. Green color represents a significant (P < 0.05) decrease in the carbonylation level and red color represents significant (P < 0.05) increases. C: control; GSE: grape seed polyphenols extract; FOM: fish oil mixture.