RgIA4 accelerates recovery from mechanical allodynia. (A) Study timeline. Sprague Dawley (SD) rats (n = 8) were treated with either vehicle (intraperitoneally (IP))-saline (subcutaneously (SC)), 8 mg/kg paclitaxel (PTX) (IP)-saline (SC), 8 mg/kg paclitaxel (IP)-RgIA4 (80 μg/kg; SC), or 8 mg/kg paclitaxel (IP)-RgIA4 (16 µg/kg; SC). Animals were tested for behavior prior to the first dose of paclitaxel (baseline (BL)) and over the course of 51 days. (B) Testing results from Von Frey assay. Results are expressed as tactile threshold values in grams (g). Black circles: vehicle-saline; red squares: PTX-saline; blue triangles: PTX-RgIA4 (16 µg/kg); hollow green triangles: PTX-RgIA4 (80 µg/kg). Mean +/- SEM are indicated. Two-way ANOVA was conducted followed by Bonferroni’s multiple comparison test, alpha = 0.05. Asterisks denote a significant difference from the PTX-Saline curve (* p < 0.05, ** p < 0.01, and **** p < 0.0001).