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. 2020 Feb 15;8:21. doi: 10.1186/s40168-020-0790-7

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — HCR-FISH fluorescence (green fluorescence, 530/40 nm band-pass filter) of four isolates treated with different fixatives. The median signal of the population from flow cytometric analysis is shown. The dashed line indicates the level of background noise. NA = not analyzed