Table 2.

Fixatives and fixation times used on pure cultures

	Fixative	Concentration	Temperature	Time
1	Formaldehyde	4%	Room temperature	1 h
2	Formaldehyde	1%	Room temperature	1 h
3	Formaldehyde	0.25%	Ice	10 min
4	Formaldehyde	0.1%	Ice	10 min
5	Ethanol	70%	Room temperature	1 h
6	Lugol’s solution	2%	Room temperature	1 h
7	Lugol’s and sodium thiosulfate	2% and 0.07 M	Room temperature	1 h
8	Glyoxal	4%	Room temperature	1 h
9	Glyoxal and ethanol	4% and 5%	Room temperature	1 h
10	Control	–	–	–