Figure 2.

A) The morphological transition of iPSCs cultured in MSC induction medium from round colonies to single spindle-shaped cells. B) Flow cytometry analysis for the cell surface markers CD73, CD105, and CD90. C) Sulfated glycosaminoglycans (red) and (D) Collagen II ( brown) were observed in pellet culture of chondrogenically differentiating iPS-MPs when cultured with TGFβ3 (10 ng ml⁻¹), BMP2 (100 ng ml⁻¹), or their combination. Scale bar is 100μm. E) Quantification of sGAG content per pellet in culture media without growth factors (CM) or with TGFβ3, BMP2, or their combination. Data are represented as mean with standard deviation (n=3).