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. Author manuscript; available in PMC: 2020 Nov 19.
Published in final edited form as: Biomater Sci. 2019 Nov 19;7(12):5388–5403. doi: 10.1039/c9bm01081e

Figure 4.

Figure 4.

IPS-MP-laden hydrogels cultured without and with loading and with TGFβ3, BMP2, and TGFβ3+BMP2 for 21 days. A.) Gene expression data for chondrogenic markers SOX9, ACAN, COL2A1 at day 21 normalized to pre-encapsulated iPS-MPs cultured under free swelling (black) and loading (gray). Representative confocal microscopy images of the ECM proteins (B) aggrecan (red), (C) collagen II (green) and nuclei (blue) and quantitative analysis of the fraction of collagen II positively stained cells of iPS-MPs cultured under free swelling (black) or loading (gray). Scale bar is 20 μm. Data are presented as mean with standard deviation (n=3).