Figure 6:

Rimantadine causes cell cycle arrest in HPV E5-expressing human head and neck cancer cell lines and inhibits cell cycle regulatory proteins. A) BrdU cell cycle was analyzed 24 hours following treatment with rimantadine. B) Stathmin mRNA expression was analyzed 24 hours following treatment with rimantadine. C) Effect of rimantadine on human HNSCC lines. BrdU cell cycle analysis (upper) and MTT proliferation assay (lower). Cells were treated with rimantadine for 24 or 48 hours for BrdU assay or MTT assay, respectively. D) Cell cycle and proliferation assay of E5-expressing cell lines. Cells were treated with rimantadine for 24 hours for BrdU assay and qPCR analysis, or for 48 hours for MTT assay at the concentration as indicated. Data are shown as mean ± S.E.M.