Figure 2.

Isolated recombinant human CAII enzyme characterization and reactivity with nitrite. (a) Human CAII purity visualized with Coomassie blue stained SDS‐PAGE. The three lanes include MW (MW ref.), dilute human CAII, and concentrated human CAII respectively. A roughly 29‐kDa band is prominent in both human CAII lanes, in agreement with the expected size of recombinant human CAII. (b) The p‐NPA hydrolysis activity assay conducted in 50‐mM phosphate buffer with 400‐μM 4‐NPA at 25°C and pH 7.5 using 1‐μM human CAII. (c) Raw data obtained by the measurement of NO using chemiluminescence during the reaction of human CAII and nitrite