Figure 5.

Carbonic anhydrase II does not affect erythrocytic nitrite‐mediated platelet activation. Nitrite (10 μM) was added to platelet rich plasma (PRP) and deoxygenated RBC (15% Hct) and allowed to incubate for 5 min before platelets were activated by ADP at 37°C. (a). Platelet activation assay presented in terms of % of activated platelets (PAC‐1 positive events) in total platelets (CD61 positive events). (b) Flow cytometry detection of platelets treated with nitrite together with RBCs (80% deoxygenated). M1, population of non‐activated platelets; M2, population of activated platelets. No significant differences were found among WT, Het, and KO RBCs. Data are presented as mean ± SEM (n = 8 per group). # P < .05, significantly different as indicated