Figure 2.

Sulfhaemoglobin assay. (a) Spectrophotometry‐derived absorbance spectrum of oxyhaemoglobin and sulfhaemoglobin. (b) Equations for the calculation of absorbance for (1) one and (2) an infinite number (n) of dissolved chromophores. (c) Extinction coefficients for deoxy Hb, oxy Hb, and sulf Hb (Zwart et al., 1981). Coefficients required for calculation of total‐ and sulfhaemoglobin are shown in shaded cells. λ, wavelength. Deoxy Hb, deoxyhaemoglobin; Oxy Hb, oxyhaemoglobin; Sulf Hb, sulfhaemoglobin