Figure 6.
Kv7.4 channels are persulfidated in malignant hyperthermia‐susceptible (MHS) biopsies. (a) The mRNA levels of Kv7.4 are not significantly different between malignant hyperthermia‐negative (MHN) and MHS biopsies, n = 5 for MHN and n = 6 for MHS. (b) Western blotting reveals no significant difference between MHN and MHS biopsies, n = 7 for MHN and n = 8 for MHS. (c) Persulfidation analysis shows that only in MHS biopsies Kv7.4 S–SH is detected, and no band has been observed in MHN biopsies. DTT, a reducing agent, has been used to eliminate the modification prior to detection. Data shown are the individual values with means ± SEM from n = 6 experiments in each group. *P < .05, significantly different as indicated. (d) Evaluation of CBS‐derived polysulfides (H2Sn) levels in tissue homogenates reveals a significant increase in MHS samples compared with MHN samples. Data shown are the individual values with means ± SEM; n = 6 for each group. *P < .05, significantly different as indicated