Figure 5.

Simultaneous silencing or pharmacological inhibition of CSE and CBS increases calcification of VIC. VIC were cultured in growth medium or calcification medium. Cystathionine‐γ‐lyase (CSE) and CSE/CBS (cystathionine‐β‐synthase) double gene silencing using siRNA was performed. (a) Calcium content, (b) CSE and CBS levels of VIC after silencing of CSE are shown. Panel (c) shows the calcium content of CSE/CBS double silenced samples. Panel (d) shows the calcium contents of pharmacological inhibition of CSE and CBS. (e) Calcium depositions of CSE/CBS double silenced VIC were shown. Data shown are means ± SEM of five independent experiments. *P < .05, significantly different as indicated; ns, not significant