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Hydrogen sulfide donors leads to inhibition of phosphate transport. Cells were cultured in a normal or calcific environment exposed to AP72 (20 μmol·L−1) for 5 days. (a) Phosphate content was determined using QuantiChrom quantitative colorimetric assay. (b) Pit1 and (c) Pit2 western blotting was performed and normalized to GAPDH. Data shown are means ± SEM of five independent experiments. *P < .05, significantly different as indicated; ns, not significant
