Figure 2.

3‐MST activity is required for basal and VEGF‐stimulated EC migration. (a) Transwell migration assay was used to assess the basal migration capacity of wild‐type, shNT, and sh3‐MST #2 ECs across chamber inserts. ShRNA‐mediated 3‐MST #2 silencing significantly decreased EC basal migration ability. (b) The addition of I3MT‐3 (100 μM) significantly inhibited the migration of shNT, but not sh3‐MST #2 ECs. Migration of shNT or sh3‐MST #2 vehicle‐treated ECs was set to 100%, and the other migration measurements were normalized to shNT or sh3‐MST #2 values. (c) 3‐MP, at low concentrations, facilitated migration of wild‐type and shNT ECs only. Migration values for wild‐type ECs in the absence of 3‐MP were set to 100%, and all other measurements were normalized to this control value. (d) VEGF‐stimulated EC migration, shNT cell migration was stimulated by both concentrations of VEGF, whereas only 30 ng·ml⁻¹ of VEGF induced a moderate migration increase in sh3‐MST ECs compared to vehicle, with less of an overall increase compared with that observed in shNT ECs. Data are shown as mean ± SEM of five independent experiments (n = 5); *P ≤ 0.05, significantly different from shNT or wild‐type group; #P ≤ 0.05, significantly different from sh3‐MST #2 group: one‐way ANOVA followed by Dunnett's post hoc test or Student's unpaired t‐test.