TABLE 1.
Primer sequences
| Gene | Forward and reverse primers |
|---|---|
| GAPDH | 5’- ACA ACT TTG GTA TCG TGG AAG G -3’ |
| 5’- GCC ATC ACG CCA CAG TTT C -3’ | |
| IL-1A | 5’- AGA TGC CTG AGA TAC CCA AAA CC -3’ |
| 5’- CCA AGC ACA CCC AGT AGT CT -3’ | |
| IL-6 | 5’- ACT CAC CTC TTC AGA ACG AAT TG -3’ |
| 5’- CCA TCT TTG GAA GGT TCA GGT TG -3’ | |
| IL-8 | 5’- ACT GAG AGT GAT TGA GAG TGG AC -3’ |
| 5’- AAC CCT CTG CAC CCA GTT TTC -3’ | |
| TNF | 5’- GAG GCC AAG CCC TGG TAT G -3’ |
| 5’- CGG GCC GAT TGA TCT CAG C -3’ | |
| ALP | 5’- CCA CAA GCC CGT GAC AGA -3’ |
| 5’- GCG GCA GAC TTT GGT TTC -3’ | |
| COL 1 | 5’- GCC CTG TCT GCT TCC TGT A -3’ |
| 5’- TTT GGG TTG GTT GTC TGT TT -3’ | |
| Runx 2 | 5’- CTT CAT TCG CCT CAC AAA C -3’ |
| 5’- GTC ACT GCG CTG AAG A -3’ |
GAPDH: Glyceraldehyde-3-phosphate dehydrogenase, IL1A: Interleukin-1α, IL6: Interleukin 6, IL8: Interleukin 8, TNF: Tumour necrosis factor, ALP: Alkaline phosphatase, COL1: Collagen type 1, RUNX2: Runt-related transcription factor 2