Table 3. Potential epitopes of five selected outer membrane proteins from Shigella flexneri 2a that can elicit both B-cell and T-cell immune response.

Protein	B-cell epitope	Position	Length (aa)	B-cell epitope Vaxijen score	No. of promiscuous T-cell epitope	No. of bound HLA alleles
						HLA-I	HLA-II
fepA	GVAQAQEPTDTPVSHDDTIVVTAA	18–41	24	0.4103	15	28	10
	MPGVNLTGNSTSGQRGNNRQIDIRGMGPEN	74–103	30	1.8893	15	21	10
	LIDGKPVSSRNSVRQGWRGERDTRGDTSW	107–135	29	1.2319	17	26	8
	YGNGAAGGVVNIITKKGSGE	155–174	20	1.3339	10	17	45
	PEHKEEGATKRTNFSLTGPLGDEF	186–209	24	0.9356	15	25	1
	GHQSARAGTYATTLPAGREGVINKDI	230–255	26	0.6457	16	32	2
	GGWDNGVTTSNWVQYEHTRNSRIPEGLAGGTEGKFNEKATQ	317–357	41	1.0477	28	38	25
	IVTPGLRFDHHSIVGNNWSPALNISQGLGDDF	447–478	32	0.8752	18	30	15
	TNPNYILYSKGQGCYASAGG	496–515	20	0.6557	10	17	18
	GQNAVGTDLYQWDNVPKAVVEG	566–587	22	0.5469	10	29	13
	YMLKSENKTTGDRLSIIPEYT	608–628	21	1.0732	11	20	1
	STLSWQAREDLSMQTTFTWYGKQQPKK	631–657	27	0.5303	15	25	11
	QQPKKYNYKGQPAVGPETKEISPYSI	653–678	26	0.8237	17	26	5
	DKRLWRAGNAQTTGDLAGANYIAGAGA	702–728	27	0.9732	15	24	1
ompC	KGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK	62–102	41	0.7724	29	31	10
	PEFGGDTYGSDNFMQQRGNGFAT	129–151	23	0.9914	9	19	9
	FAVQYQGKNGSPEGEGMTNNG	166–186	21	1.1887	9	13	28
	NRYDERYIGNGDRAETYTGGLKYDANNI	228–255	28	0.7238	13	27	1
nlpD_1	GMLITPPPKMGTTSTAQQPQIQPVQQ	50–75	26	0.7286	16	26	16
	QPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSYSGSTY	83–123	41	0.4008	27	34	29
	NVGQTLQVGNASGTPITGGNA	158–178	21	1.1147	6	13	2
	QGVVIKPAQNSTVAVASQPTITYSESSGEQSAN	187–219	33	0.8033	22	30	41
	WPTEGKVIETFGASEGGNKGIDIAGSKG	262–289	28	1.0396	15	21	2
tolC	DYTYSNGYRDANGINSNATSASLQLTQ	69–95	27	1.1696	16	25	21
	PELAALNVENFKTDKPQPVNALLK	213–236	24	0.5435	15	37	11
	ASTGISDTSYSGSKTRGAAGTQYDDSNMGQN	274–304	31	1.1866	12	18	2
	PIYQGGMVNSQVKQAQYNFVGAS	313–335	23	0.5065	12	25	31
	DLLALNNALSKPVSTNPENVAPQT	433–456	24	0.5650	14	27	16
	QTPEQNAIADGYAPDSPAPVVQQ	455–477	23	0.4310	11	25	2
nlpD_2	GLLLAGCSGSKSSDTGTYSGSV	28–49	22	1.2578	6	14	20
	SPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTTKT	79–112	34	1.3560	23	34	30

In immunogenic sequences with more than one B cell epitope predicted, N-terminals of predicted B epitope sequences are underlined.

HLA, human leukocyte antigen.