Figure 1.

SRV seroreactivity patterns of baboons with unclear infection status. Western blots demonstrate the SRV antibody reactivity of the antibody reactive (positive or indeterminate), but PCR negative baboon donors from which PBMC were initially cultured in an attempt to isolate a virus. The target antigen on the blots is a mixture of SRV1 and SRV2 viral lysate. Each strip was reacted with either serum of an SRV positive macaque control (+) or of individual baboons that were used for the PBMC culture experiment (1-6). All the baboon sera reacted against the transmembrane gp20 and core p27. Although there is not clear reactivity to env gp70 this pattern is sufficient to interpret as indeterminate or positive in most routine SRV testing. *Hypothesized due to reactivity of these bands to mAb specific for gp20.