Figure 2.

In vitro infection experiment of baboon PBMC. (A) SRV PCR remained positive for both baboon PBMC cultures inoculated with SRV1 and (B) for one of 2 baboon PBMC cultures inoculated with SRV2, even after the cells were washed and subcultured into fresh cells and media. Rhesus macaque (MMU) PBMC were also inoculated as positive controls. SRV DNA qPCR results are shown as the ratio of cycling threshold (Ct) values for SRV DNA normalized against the Oncostatin M (OSM) internal housekeeping gene Ct values in the same sample and plotted over time. SRV Ct values greater than 55 are interpreted as negative for SRV DNA. A decline in Ct ratio of SRV/OSM, as indicated by the * on the graph, indicates increasing amounts of SRV DNA.