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. 2020 Feb 12;13:447–454. doi: 10.2147/IDR.S239850

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© 2020 Guo et al.

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Efflux pump gene expression by clinical, M. abscessus isolates following exposure to CLA stress. Isolates that were CLA-susceptible, -intermediate and resistant [without (unexplained) or with a rrl 2270/2271 mutation] are shown. MAB_2355c (A), MAB_1409c (B), MAB_1846 (C) and whiB7 (D), message levels were quantified by qRT-PCR. Open circles, triangles, diamonds, and solid circles indicate the distribution of mRNA expression levels in isolates comprising the sensitive, intermediate, unexplained drug resistance, and rrl 2270/2271 mutation groups, respectively. Experiments were repeated >3 times for each isolate; error bars represent the standard error of the mean for each data set.