Table 1.
Copy number, concentration, and membrane density of signaling proteins
| Proteins | Molecules/Flagelluma | Concentrationb (μM) | Densityc (molecules/μm2) |
|---|---|---|---|
| GC | 400,000 | 400 | 11,976 |
| CNGK | 21,653 ± 4,038 | 21.7 ± 4 | 648 |
| sNHE | 53,979 ± 20,659 | 54 ± 20.7 | 1,616 |
| sAC | 6,718 ± 2,630 | 6.7 ± 2.6 | 201 |
| HCN1 | 10,258 ± 901 | 10.3 ± 0.9 | 307 |
| HCN2 | 18,337 ± 887 | 18.3 ± 0.9 | 549 |
| CatSper1 | 1,853 ± 147 | 1.9 ± 0.1 | 56d |
| CatSper2 | 2,125 ± 138 | 2.1 ± 0.1 | |
| CatSper3e | 587 ± 296 | 0.6 ± 0.3 | |
| CatSper4 | 1,208 ± 113 | 1.2 ± 0.1 | |
| CatSper β | 1,690 ± 547 | 1.7 ± 0.5 | |
| CatSper γ | 1,169 ± 1,395 | 1.2 ± 1.4 | |
| CatSper δ | 2,071 ± 392 | 2.1 ± 0.4 | |
| PDE5 | 48,567 ± 6,525 | 48.6 ± 6.5 | |
| PDE10 | 4,211 ± 752 | 4.2 ± 0.8 | |
| NCKXe | 120,795 ± 41,520 | 120.8 ± 41.5 | 3,617 |
| Ca2+‐ATPase | 1,991 ± 749 | 2 ± 0.7 | 60 |
| Na+/K+‐ATPase | 48,280 ± 16,788 | 48.3 ± 16.8 | 1,446 |
| Creatine Kinase | 364,465 ± 29,428 | 364.5 ± 29.4 | 10,912 |
a
Mean ± SD from six animals.
b
Calculated from column 2, assuming a matrix volume V ma of 1.7 femtoliter.
c
Calculated from column 2, assuming a flagellar surface area of 33.4 μm2 (r = 0.133 μm; l = 40 μm); only membrane‐bound proteins are considered.
d
Mean calculated from all CatSper subunits, except CatSper3, assuming a 1:1 stoichiometry of subunits.
e
Data from a single peptide only.