Figure 4.

The anti-oncogene roles of ROCK1 silencing are mediated by XIST/miR-139-5p.

Notes: (A) Binding sequence between miR-139-5p and ROCK1-3′UTR region. (B) Luciferase activity was detected in HS-1 cells co-transfected with ROCK1-wt or ROCK1-mut reporter and miR-139-5p mimic, mimic NC, miR-139-5p+vector, or miR-139-5p+pcDNA-XIST. (C and D) Protein level of ROCK1 in HS-1 cells with miR-139-5p overexpression, XIST knockdown, or miR-139-5p inhibitor. All experiments were performed in triplicate and repeated three times. (E and F) Correlation assay between ROCK1 and miR-139-5p or XIST in melanoma tumor tissues. *P<0.05, **P<0.01.

Abbreviations: WT, wild type; MUT, mutant type; miR-NC, scrambled RNA for negative control.