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. 2020 Feb 5;2020:6318520. doi: 10.1155/2020/6318520

Figure 2.

Figure 2

AE inhibits PA-induced inflammatory cytokine production in H9C2 cells. (a–c) H9C2 cells pretreated with different concentrations of AE for 1 h and incubated with 500 μM PA for 14 h. The total RNAs were extracted from cells for real-time qPCR analysis as shown in (a)–(c). All the genes were normalized with GAPDH as a control (n = 6). (d, f) The cells were harvested and processed to immunofluorescence staining of vascular adhesion factor (ICAM-1 and VCAM-1) as described in the Material and Methods section. (e, g) Representative group fluorescence intensity was shown. Transverse line = 100 μm (n = 4; ∗∗∗P < 0.001 compared to the control group; ##P < 0.01 and ###P < 0.001 compared to the PA group).