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. 2019 Dec 27;11(2):133–140. doi: 10.1021/acsmedchemlett.9b00493

Figure 6.

Figure 6

Mode of inhibition against SMYD3 by isoxazole series compounds. IC50 values were determined following a 60 min SMYD3 reaction and fitting the data to a 3-parameter dose–response equation. Top Panel: Data represents independent experiments showing IC50 values plotted as a function of [FL MEKK2]/Kmapp for compound 7 (A), 28 (B), and 49 (C, n = 1) where IC50 = Ki for noncompetitive inhibition. Bottom Panel: Data represents independent experiments showing IC50 values plotted as a function of [SAM]/Kmapp fit to an equation for uncompetitive inhibition αKi = IC50/(1 + (Km/[S])) for compound 7 (D), 28 (E), and 49 (F, n = 1).