Table 1.

Identified peak pairs (tier 1 and tier 2) that were affected by dietary supplementation of a blend of S. cerevisiae-based direct-fed microbials and fermentation products

Compound	Fold change	FDR
Galactose	2.60	< 0.01
Lactose	0.46	< 0.01
Glucose	2.62	< 0.01
Fructose	2.31	< 0.01
Isomer of fructose	2.30	< 0.01
Isomer of glyceraldehyde	2.01	0.01
Glyceraldehyde	2.01	0.01
Hippuric acid	2.13	< 0.01
Phenylacetylglycine	1.98	0.01
5-Hydroxykynurenamine	2.63	< 0.01
4-Oxoglutaramate	1.82	< 0.01
2-Dehydro-3-deoxy-D-glucarate	1.80	< 0.01
3-Fumarylpyruvate	2.58	< 0.01
1-Deoxy-D-xylulose 5-phosphate	2.36	< 0.01
Glycolaldehyde	1.63	0.01
Hydroxypyruvate	1.60	< 0.01
2-dehydro-3-deoxy-L-arabinonate	0.30	< 0.01
Acetoacetate	0.62	0.01
Dehydroascorbate - 2 tags	1.74	0.01
3-Methylindolepyruvate	3.72	< 0.01
(S)-2-Aceto-2-hydroxybutanoate	2.96	< 0.01
5-Oxopentanoate	2.30	< 0.01
(R)-3-Hydroxy-3-methyl-2-oxopentanoate	2.96	< 0.01
2-Dehydropantoate	3.28	< 0.01
Isomer of (S)-2-aceto-2-hydroxybutanoate	3.51	< 0.01
Isomer of (S)-3-methyl-2-oxopentanoic acid	2.19	< 0.01

FC: fold change relative to control

P-value was calculated from student’s t-test

Tier 1 - Positive identification (chemical isotope labelling library);

Tier 2 - High confidence putative identification (linked identity library library)

Only metabolites with both fold-change ≥1.5 or ≤ 0.67 and FDR ≤ 0.01 are shown