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. 2020 Feb 14;19:1534735419901160. doi: 10.1177/1534735419901160

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© The Author(s) 2020

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 6. — Apoptotic potential of thymoquinone (TQ) using the annexin-V assay. (A) Flow cytometric analysis of both proliferative and Dox-treated HCT116 and MCF7 cells. Red color represents the live cells, and green color represents the annexin-V–positive cells. (B) The percentages of annexin-V–positive cells of proliferative HCT116 and Dox-treated HCT116. (C) The percentages of annexin-V–positive cells of proliferative MCF7 and Dox-treated MCF7. Cells were exposed to TQ (0, 10, 25, 50, and 75 µM) for 24 hours. TQ (0 µM) is a control for the corresponding proliferative or senescent of either HCT116 or MCF7. Data of all concentrations were subtracted by the data of 0 µM. Data were analyzed with 2-way ANOVA followed by Bonferroni posttest. *P < .05, **P < .01, and ***P < .001 versus TQ 0 µM. ⁺P < .05 and ⁺⁺⁺P < .001 versus TQ 10 µM. ^xxP < .01 and ^xxxP < 0.001 versus TQ 25 µM. ^###P < .001 versus TQ 50 µM for HCT116, HCT116-Dox, MCF7, and MCF7-Dox, separately. ^ΘΘΘP < .001 versus TQ 25 µM in proliferative and Dox-treated cells. ^ФP < .05 and ^ФФФP < .001 versus TQ 50 µM in proliferative and Dox-treated cells. ^θθP < .01 and ^θθθP < .001 versus TQ 75 µM in proliferative and Dox-treated cells. Error bars represent mean ± SD. PE-annexin-V signals were observed at excitation/emission 496, 546, and 565⁄578 nm. Control refers to proliferative cancer cells. HCT116-Dox refers to control senescent HCT116 cells. MCF7-Dox refers to control senescent MCF7 cells. White bars refer to proliferative cancer cells. Black bars refer to senescent cancer cells.